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Paxton and Harris  also concluded that enhanced levels of BCKA could inhibit the BCKDH kinase, which results in a reduced phosphorylated BCKDH complex and therefore a higher BCKDH activity.
In recent years, increased attention has been paid to the metabolism and biological functions of the branched-chain keto-acids (BCKAs)  i.e., [alpha]-ketoisocaproate (KIC), [alpha]-ketoisovalerate (KIV), and [alpha]-keto-[beta]-methylvalerate (KMV).
The first purpose of this study was to develop, for clinical use, a reliable HPLC technique with fluorometric detection based on quinoxalinol derivatives for the determination in serum of BCKAs and pyruvate, using o-phenylenediamine (OPD) as a fluorogenic agent.
The quinoxalinol derivatives of BCKAs were detected by monitoring the fluorescence emission at 410 run (with excitation at 350 nm).
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- Bcl 2
- Bcl 2
- BCL-1 oncogene
- bcl-1, PRAD1