Analysis of variance revealed that the 28 entries were significantly different (P [less than or equal to] 0.05) in their response to field diseases; angular leaf spot (ALS), anthracnose (ANT), common bacterial blight (CBB), bean common mosaic virus disease (BCMV
), bean common mosaic virus necrosis disease (BCMNV)/black rot (BR), bean rust, and yield.
Gel picture showing amplification of partial CP gene in BCMV
infected Leaf samples
Each plant sample was placed separately in a plastic bag and kept at 4[degrees]C until analyzed by Bean yellow mosaic virus (BYMV), Bean common mosaic virus (BCMV
), Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Alfalfa mosaic virus (AMV) polyclonal antibodies, and DAS-ELISA was performed as described by Clark and Adams .
For currently, no single commercial bean cultivar offers combined resistance to all races of rust fungus and all strains of BCMV
, says plant pathologist J.
Silver Cloud has dominant I resistance to BCMV
and complete resistance to CTV.
The presence of the SCAR marker SW13 suggested that PR9745-232 has the I allele for resistance to Bean common mosaic virus (BCMV
), a potyvirus.
They also possess more favorable alleles and QTL that impart higher level of resistance to drought (Teran and Singh, 2002), low soil fertility (Singh et al., 2003), BCMV
and BCMNV (Drijfhout, 1978), common bacterial blight (Singh and Munoz, 1999), halo blight (Taylor et al., 1978, 1996b), and other biotic and abiotic stresses (see review by Singh, 2001).
In 2004, Carrizalito was mechanically inoculated at the University of Puerto Rico (UPR) with the NL3 strain of Bean common mosaic necrotic virus (BCMNV) and found to have top necrosis caused by the presence of the dominant I gene for resistance to BCMV
. Ashy stem blight is favored by warm dry conditions associated with drought stress (Mayek-Perez et al., 2002).
Flor de Mayo 2000 carries the single dominant hypersensitive Igene for resistance to Bean common mosaic virus (BCMV
) but is sensitive to the necrosis-inducing strains of Bean common mosaic necrosis virus (BCMNV), which induce the black root reaction.
phaseolicola (Burkholder 1926) Young, Dye & Wilkie 1978], Bean common mosaic virus (BCMV
, potyvirus), and Bean common mosaic necrosis virus (BCMNV, potyvirus).