Several cases of BCPI infarctions have been reported previously associated with locked-in syndrome.
reported a study of 14 cases of BCPI, where 12 (85.7%) patients had vertebrobasilar artery severe stenosis or occlusion.
It was different from previous reports in which BCPI were associated with locked-in syndrome and disturbance of consciousness.
Studies with other inhibitors, crammer and BCPI, were also performed.
Recombinant BCPI , recombinant CTLA-2[alpha] , and recombinant crammer  were purified according to the methods described previously.
For inhibition studies of crammer and BCPI, Bombyx cysteine protease (BCP) was employed.
The recombinant inhibitor proteins used in this experiment, CTLA-2[alpha], crammer, and BCPI, can be seen in Figure 2(a), as homogeneous forms in the Tricine/SDS-PAGE gel .
Similar to BCPI, the inhibition of CTLA-2[alpha] was not dependent on pH, in a range between pH 4.0 and pH 6.0 .
Alanine Scanning of Crammer and BCPI. The Drosophila CTLA-2-like protein, crammer, has a cysteine residue (C72) at the homologous region to CTLA-2[alpha], whereas Bombyx BCPI does not (Figure 1).