Several cases of BCPI infarctions have been reported previously associated with locked-in syndrome.
reported a study of 14 cases of BCPI, where 12 (85.7%) patients had vertebrobasilar artery severe stenosis or occlusion.
It was different from previous reports in which BCPI were associated with locked-in syndrome and disturbance of consciousness.
Studies with other inhibitors, crammer and BCPI, were also performed.
Recombinant BCPI [4], recombinant CTLA-2[alpha] [11], and recombinant crammer [7] were purified according to the methods described previously.
For inhibition studies of crammer and BCPI, Bombyx cysteine protease (BCP) was employed.
The recombinant inhibitor proteins used in this experiment, CTLA-2[alpha], crammer, and BCPI, can be seen in Figure 2(a), as homogeneous forms in the Tricine/SDS-PAGE gel [27].
Similar to BCPI, the inhibition of CTLA-2[alpha] was not dependent on pH, in a range between pH 4.0 and pH 6.0 [4].
Alanine Scanning of Crammer and BCPI. The Drosophila CTLA-2-like protein, crammer, has a cysteine residue (C72) at the homologous region to CTLA-2[alpha], whereas Bombyx BCPI does not (Figure 1).