BFOIBond Forfeited, Order In (Franklkin County Municipal Court; Columbus, OH)
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The single nucleotide polymorphism (SNP) in the regulatory region of the IGF1 gene was detected by amplification of the 294 bp region using specific primers and cleavage with the BfoI enzyme.
For IGF1/BfoI genotyping, 10 ul of PCR product were digested with 2 ul (20 U) of Fast Digest BfoI (FD2148, ThermoFisher) restriction enzymes at 37AdegC for 5 min.