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Moreover, areas where the cell integrity is affected, that is, areas where the cortical ring-shaped actin on cell borders disappears (arrows) are visible under mixed exposure (hypoxia + AZA) conditions, further confirming DIC and BFRL data.
By evaluating cell morphology (DIC), cell surface contacts (BFRL), and the actin cytoskeleton (fluorescence), as well as the extracellular medium acidification (pH measurements), we were able to confirm the cellular effects induced by hypoxic conditions and upon AZA inhibition of CA highlighted noninvasively and label free using impedance bioassay.
These components, along with ongoing work in BFRL and MEL, will be integrated in the NIST Distributed Testbed for First Responders in the next several months.
NIST was asked to intervene and Eberhardt led in the evaluation of screening test conducted by the Fire Science Division of the Building and Fire Research Laboratory (BFRL).