Restriction fragment length polymorphism (RFLP) analysis of the PCR products of the open reading frame (ORF) 38 and ORF54 loci using PstI and BgII
(5) demonstrated that the strain had a vOka-like pattern, i.e., PstI-BgII+.
The PCR was followed by BgII
or KasI restriction endonuclease digestion to determine the genotype of the -201CT and -360CG SNVs, respectively.
In 1999, BGII
Technologies received BLACK ENTERPRISE magazine's Emerging Company of the Year award.
For an initial assessment of rDNA variation in cotton, approximately 3 to 5 [micro]g of DNA from 16 accessions-cultivars (Table 1) was restriction digested with the enzymes BamHI, BgII
BstEII, DraI, EcoRI, EcoR V, HindIII, KpnI, NcoI, NdeI, NsiI, SacI, ScaI, SpeI, SspI, XbaI, and XhoI.
Aliquots of mtDNA from each fish were digested separately with 12 restriction endonucleases: four hexameric (PvuII, ApaI, BgII
, DraI), six multi-hexameric (AvaI, BanI, BanII, HaeII, HindII, StyI), and two multi-pentameric (NciI, AvaII) enzymes.