Standard volumes (three 0.025 mL drops) of the fluid samples, tissue homogenates, and all dilutions were used to drop-inoculate the surface of two bacteriological media poured in Petri dishes, brain heart infusion agar (BHIA
; Difco) and R2A agar (Difco).
difficile, subcultures were made from cooked meat broth onto CCFA and BHIA
and incubated anaerobically at 37[degrees]C up to 5 days before being discarded as negative.
MRSA ATCC 43300 was treated with tannins at 0.78 mg/mL prior to inoculation on BHIA
after 24 h incubation at 37[degrees]C.
In her nomination letter, Kathleen Ogden, president of the Boston Society of Landscape Architects, praised Abbott's six and a half-year tenure at the BHIA
Sahib Ji, patron and chairman of the Nishkam School with Richard Dakin, director of Bam Construction in the Midlands
ruckeri can be isolated after cultivation in culture media such as TSA, BHIA
, ROD and SW, by incubating for 48 hours at 22-25oC.
Forty nine positive isolates were subcultured on CBA and BHIA
media supplemented with 5% defibrinated sheep blood and Dent antibiotic supplement.
fusidico y carbenecilina) y el de Dentaid-1 (21) (BHIA
To isolate fungi, the specimens were inoculated on to Sabouraud dextrose agar (SDA) and brain heart infusion agar (BHIA
) and incubated at 25[degrees]C and 37[degrees]C.
Bits of these organs and tissue were macerated and cultured on brain heart infusion agar (BHIA
) slants supplemented with chloramphenicol (0.05 mg/mL) and incubated at 30[degrees] C for up to 4 weeks.
Biopsies (2 each from the antrum and corpus) were taken, submerged in 5% brain-heart-infusion agar (BHIA
), enriched with 5% fetal bovine serum (FBS) and kept at 4[degrees]C till processing.
In the north Bhia
valley the last stronghold of terrorists has been fully secured and in the west, area of Shamozai is being cleared.