BHIBBrain-Heart Infusion Broth
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Briefly, 1ml of overnight bacterial growth on BHIB was placed into 1.5ml microcentrifuge tubes and centrifuged at 10000 rpm for 1 minute.
Gram staining has been done with bacteria in BHIB bacterial suspension, and gram-negative bacteria is obtained in the form of bacil composed of monobacil.
* After incubation mixed in a test tube 0.5 ml human plasma + 0.5 ml of culture on BHIB.
BHIB broths with 2.5% foetal bovine serum (10 mL) containing [S.sub.5] at concentrations of 1.0 [micro]gm[L.sup.-1], 2.0 [micro]gm[L.sup.-1], 4.0 [micro]gm[L.sup.-1], 8.0 [micro]gm[L.sup.-1], and 16 [micro]gm[L.sup.-1] were inoculated with 10 [micro]L of the fresh bacterial culture and shaken at 37[degrees]C in a microaerophilic atmosphere.
Enterococcus faecalis, obtained from American Type Culture Collection (ATCC) 29212, was cultured at 37[degrees]C for 24 hours in BHIB. 0.5 mL aliquots of the E.faecalis culture were used to inoculate the upper chambers using a micropipette.
A colony of each isolate was inoculated into tubes containing two ml of brain heart infusion broth (BHIB) and incubated at 37[degrees]C for 24 hours.
coli O157: H7 inoculated on BHIB medium liquid are incubated 18 hours at 37 [degrees]C; while the lactic acid bacteria are inoculated in MRS spot on solid buffered in order to eliminate the effect of lactic acid (pH 5.4) then they were incubated 24 h at 30[degrees]C.
were cultured in nutrient broth (NB) (Merck) at 37[degrees]C; Streptococcus pneumoniae ATCC 49617, Enterococcus faecalis ATCC 29212, and Enterococcus faecalis ATCC 51299 were cultured in brain heart infusion broth (BHIB) (Merck) at 37[degrees]C for 24 h.; Micrococcus luteus ATCC 9341, Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 6633, and Bacillus thuringiensis were cultured in nutrient broth (NB) (Merck) at 30[degrees]C; Candida utilis, Candida albicans ATCC 90028, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae ATCC 9763 were cultured in malt extract broth (MEB) (Merck, USA) at 30[degrees] C for 24 h.
Swabs taken from letters, powders, objects, clothes, and other items in the high containment BSL-3 area were plated directly on sheep blood agar (SBA) or transferred onto brain heart infusion broth (BHIB) and incubated there.
Before testing, all strains were stored at -70[degrees]C in brain heart infusion broth (BHIB, Centers for Disease Control and Prevention [CDC], Atlanta, GA) or water containing 20% glycerol.