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We found that high PLK1 expression levels were associated with depressed Treg cell enrichment levels in 16 cancer types (THYM, LUSC, GBM, TGCT, SKCM, PRAD, UCEC, ESCA, UCS, UVM, OV, DLBC, LUAD, STAD, CESC, and KICH) while were associated with enhanced Treg cell activity in 5 cancer types (THCA, KIRC, LIHC, BRCA, and BLCA) (Spearman correlation, FDR<0.1) (Figure 3).
The analysis of NMP22, BLCA, and NMBL proteins performed according to tumor stage (T) and grade (G) [Ta (n = 43), T1 (n = 31), T2 (n = 10), and TIS (n = 7); G1 (n = 40), G2 (n = 41), and G3 (n = 3)] showed no differences between subgroups T and G in NMP22 level; however, there were statistically significant differences in BLCA-4 concentration between Ta and T1 (p = 0.0115), Ta and T2 (p = 0.0288), and Ta and TIS (p = 0.0255) (Figure 1(a)).