BMMNCbone marrow aspirate mononuclear cells
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BMMNCs were incubated with biotin-conjugated rat antibodies specific for murine CD5, Mac-1, CD45R/B220, Ter-119, and Gr-1 for 15 min at room temperature.
Ratios of HPCs (LSK-) and HSCs (LSK+) in iron overload BMMNCs
BMMNCs derived from each group were grown in triplicate in M3434 semi-solid media.