Moreover, miR-124 is a negative regulator of osteoclastogenesis in mouse BMMs
. It reduces the expression of NFATc1 protein induced by RANKL stimulation and inhibits the OC precursors by targeting expression of RhoA and Rac1, GTPases involved in cellular motility .
"The mortality ratio due to eclampsia increased from 39 per 100,000 live births in BMMS
2010 to 46 per 100,000 live births in BMMS
2016 while the proportionate contribution of eclampsia to all maternal deaths increased from 20 percent to 24 percent," he added.
isolated from the tibiae of 4-week-old ICR mice were cultured with [alpha]-MEM containing M-CSF (30 ng/ml), 10% FBS, and 1% antibioticantimycotic mixture in a humidified atmosphere of 5% C[O.sub.2] at 37[degrees]C.
Furthermore, miR-16-5p levels were substantially decreased in vitro during the process of RANKL-induced osteoclastogenesis in BMMs
. Moreover, our gain of function experiment demonstrated that a miR-16-5p mimic significantly reduced RANKL-induced osteoclast formation.
were cultured in 96-well culture plates with sanguiin H-6 in the presence of M-CSF (30ng/ml) and RANKL (50ng/ml).
To study whether intraperitoneal injection of macrophages induces an acute phase response in mice, BMM
and J774 cells were prepared as described above, except that [sup.3]H-cholesterol was omitted from the media during the cholesterol loading step.
For stromal cell-free bone marrow-derived macrophage (BMM
) culture, bone marrow cells were cultured with M-CSF (50ng/mL) for 3 day in [alpha]-MEM containing 10% FBS, and attached cells were used as osteoclast precursors, BMMs
(23) The next most recent national-level estimates of abortion's role in maternal mortality date from 1998-2001, the reference period for the first of the country's two maternal mortality studies--the 2001 BMMS
. (4) That assessment indicated that just 5% of maternal deaths were related to induced abortion, a considerable decline from the 26% measured in 1978-1979--even though the latter study used a different, less rigorous methodology than was previously used.
(47) The 2001 BMMS
cited that 61% of women experienced at least one complication during pregnancy, delivery and after delivery, the most common being eclampsia and malpresentation or prolonged/obstructed labour.