The Chemistry of Bodipy
: a newel Dorado for fluorescence tools.
 Nonstandard abbreviations: MSP, methylation-specific PCR; MS-FLAG, methylation-specific fluorescent amplicon generation; LAMP, loop-mediated isothermal amplification; dNTP, deoxynucleoside triphosphate; MS-LAMP, methylation-specific LAMP; BODIPY
, dipyrromethene boron difluoride; TAMRA, 6-carboxytetramethylrhodamine.
Thatcher, "Nitroxidation, nitration, and oxidation of a BODIPY
fluorophore by RNOS and ROS," Nitric Oxide, vol.
Cells were then washed 3 times and incubated for 1h with BODIPY
493/503 dye for lipid droplet staining, DAPI for nuclear staining, and Alexa Fluor 568 goat anti-rabbit or anti-mouse IgG for PPAR[gamma] and C/EBP[beta], respectively.
The compound BODIPY
1 was synthesized similarly as the reported reference .
Therefore our results confirm that the plasma membrane C-11 BODIPY
is a suitable probe in the evaluation of the effects on the oxidative burst of flavonoids, which increase DHR123 accumulation .
Forward primers for DEN-1 and DEN-3 were labeled at the 5' end with BODIPY
630/650 (Molecular Probes/ Invitrogen) and Cy5.5, respectively.
A very interesting new generation of transition metal free CORMs based on boron-dipyrromethene (BODIPY
) chromophores (named COR-BDPs) were synthesised by Palao et al.
For confocal microscopy, larvae were permeabilized in three 10-min changes of PBS with 0.1 % Triton X-100 (Fisher Scientific), hereafter referred to as PBT, then stained with Bodipy
FL phallacidin (1U/100 [micro]l of PBT) for 40 min at room temperature.
Fluorescenn, rhodamine (Texas Red), phycobiliproteins, nitrobenzoxadiazole (NBD), acridines, Cy3, Cy5, and bodipy
compounds are commonly used for protein labeling (20, 21).
For fibrillar actin labeling, fixed cells were washed twice with PBS, incubated with 0.15 mmol/L BODIPY
FL phallacidin (Molecular Probes) in PBS containing 10 g/L bovine serum albumin (BSA) for 20 min at room temperature, washed 3 times with PBS, and mounted in SlowFade (Molecular Probes).
The antioxidant activity of Murtilla fruit extract was determined by the luminescence reaction of luminol [5-amino-2,3-dihydro-1,4-phthalazinedione] (Sigma); membrane lipid peroxidation using Bodipy
C-11 sensor (Invitrogen); and intracellular superoxide anion production using dihydroethidium (DHE, Invitrogen), a sensor of superoxide anion ([O.sub.2.sup.-]), using procedures described by the manufacturers.