Effects of BOEC co-culture system on bovine IVM/IVF embryos
The effects of different status of the BOEC co-culture system on development of bovine IVM/IVF embryos were examined.
Morphology and gene expression in different status of BOEC used or not used in embryo culture
To establish an mRNA phenotypic map for the expression of various growth factors, apoptosis and antioxidant genes, BOEC used or not used in embryo culture were analyzed according to different cell status by RT-PCR.
The expression patterns of apoptosis genes (Bax, Caspase-3, and p53) in BOEC with different cell status, which was used or not used in embryo culture, were examined (Figure 3).
The transcripts for CuZnSOD, MnSOD, Catalase, and GPx were detected in BOEC used or not used in embryo culture according to different cell generation and status (Figure 4).
According to the experimental purpose, BOEC pellets were suspended and cultured in DMEM containing 10% FBS for 2-3 days on 4-well plates (a density of 1x[10.
Repeated measures ANOVA was used to test the main effects of melatonin and SNP on cell viability and LPO, and subsequent development rates of bovine IVM/IVF embryo with BOEC co-culture.
The cell viability of BOEC in 50 [micro]M and 100 [micro]M SNP groups increased significantly compared with 1,000 [micro]M and 2,000 [micro]M SNP groups (p<0.
The cell viability, LPO and expression of apoptosis and antioxidant genes in BOEC pre-treated with melatonin (1,000 [micro]M) in the presence or absence of SNP (1,000 [micro]M) are indicated in Figure 3.
Developmental rate to blastocyst stage in the BOEC coculture system, which was pre-treated with SNP, melatonin alone or melatonin plus SNP, was 28.
facilitators lead post-activity discussions about what worked and what didn't.