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BOESBorderless Order Entry Systems (DE Technologies, USA)
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Here, we show that BoE significantly reduced the weight gain induced by a HFD.
Biochemical analysis and multiplex ELISA quantitation for sera revealed accumulation of TCHO (Table 5), strong induction of inflammatory cytokines by HFD intake (Table 6 and Supplementary Table 5), and BoE supplementation significantly reduced levels of IL-5, IL-6, IL-13, and GCSF (Table 6).
All this accumulating evidence may support the antiobesity and anti-inflammatory effects of BoE on our experimental animal model although the responsible molecules for these activities are still elusive.
Abbreviations BoE: Condensed fermentative extracts of bonito anserine: [beta]-Alanyl-N-methylhistidine DHA: D ocosahexaenoic acid EPA: Eicosapentaenoic acid HFD: High-fat diet AST: Aspartate aminotransferase ALT: Alanine transaminase LDH: Lactate dehydrogenase CPK: Creatine phosphokinase TCHO: Total cholesterol TG: Triglycerides IL: Interleukin G-CSF: Granulocyte-colony stimulating factor CT scan: Computed tomography scan RT-qPCR: Real time-quantitative polymerase chain reaction UCP-1: Uncoupling protein 1 GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.
Two doses of condensed fermentative extracts of bonito (BoE) were used as supplements.
Caption: Figure 2: Effects of BoE supplementation on body weight of mice fed a high-fat diet.
Caption: Figure 3: Weekly consumption of the high-fat diet with or without BoE. The average amount of diet consumed each week was calculated for each group.
Caption: Figure 4: Induction of body fat reduction by BoE supplementation.