Toxicity studies, for comparison of several lipoplexes and 25kDa bPEI, were performed using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) reagent (Sigma-Aldrich, Taufkirchen, Germany).
DOTAP and 25 kDa bPEI showed the most efficient complexation with pDNA, whereas all other liposomes were less effective in binding pDNA.
Superior complexation efficiency was observed in 25 kDa bPEI due to the polycationic nature of the polymer favouring particles with sizes of 60 nm (Table 2 and Figure 9(a)).
(a) 25 kDa bPEI, (b) DOTAP, (c) hGDNT/DOTAP (5: 95), (d) PLFE/DOTAP (5: 95), (e) hGDNT/CH/DOTAP (5:45:50), (f) PLFE/CH/DOTAP (5:45:50), and (g) hGDNT/PC/CH/DOTAP (5:30:35:30).
At the inception of the Argus II project, Miami Lighthouse for the Blind and Visually Impaired was chosen to partner with BPEI and Second Sight and to provide vision rehabilitation for retinal implant patients in Florida.
Six vision rehabilitation sessions totaled 44 hours, and an additional 6 hours were spent in orientation and mobility training and accompanying the patient for device adjustment at BPEI. Also, 60 hours were spent in training in California.
In the fourth session, which was eight hours in length and took place on January 26, 2015, despite an adjustment at BPEI that attempted to decrease light intensity, considerable glare was noted and further adjustment of the setting was requested.
Prior to the sixth session, the patient came to Miami for a follow-up visit at BPEI. BPEI technicians and a team from Second Sight made adjustments to the patient's Argus II system, and orientation and mobility training under the guidance of Miami Lighthouse's certified O&M specialist subsequently began.
The coating recipe based on BPEI
pH 7 and NaMt 1 wt.