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Polymerase Chain Reaction (PCR) was done to detect BPV-1 and BPV-2 viruses in wart tissues of eighteen animals (Fig.
Detection of BPV-1 and -2 and quantification of BPV-1 by real-time PCR in cutaneous warts in cattle and buffaloes.
Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids.
In silico analyses of the restrictionsites in FAP L1 fragments of 20 deposited BPVs sequences (from BPV-1 to BPV-13) revealed no intratype variation associated with the relative cut positions for the four enzymes employed (Table 1).
In a previous report , Hatama and others discussed the "uncertain nature of BPV-11 tumorigenicity" since BPV-11 was first diagnosed in a fibropapilloma lesion in which the BPV-1 was also detected.
Specifically, the BPV-1 and 2 are classified as Deltapapillomaviruses [14, 15].
The BPV-1 is commonly associated with lesions in the teats and udder [13,18,19].
Viral identification was performed using specific primers for BPV-1 (forward: 5-GGAGCGCCTGCTAACTATAGGA-3' and reverse: 5'-ATCTGTTGTTTGGGTGGTGAC-3'), which amplifies the L1 gene, resulting in a 301bp amplicon, BPV-2 (forward: 5'-GTTATACCACCCAAAGAAGACCCT-3' and reverse: 5'-CTGGTTGCAACAGCTCTCTTTCTC-3'), which amplifies the L2 gene, resulting in a 164 amplicon, and BPV-4 (forward; 5'-GCTGACCTTCCAGTCTTAAT-3' and reverse; 5'-CAGTTTCAATCTCCTCTTCA-3'), which amplifies the E7 gene, resulting in a 170 bp amplicon.
We selected the specific primers for BPV-1, -2, and -4 due to their prevalence in the herd, and we could detect the virus sequences in peripheral blood cells collected from the adult animals, with and without skin papillomas (Figure 1(a)).
coli- derived [L.sub.1] protein protected calves against BPV-1 challenge after vaccination.
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