These primers were used in a PCRreactiontoamplifyE6 gene, using the genomic DNA of BPV-1 or BPV-2 previously cloned in pAT153 vector as a template .
In the present work, the E6 gene of both BPV-1 and BPV-2 was cloned in a bacterial expression system, with the respective recombinant proteins being purified.
Both BPV-1 and BPV-2 encode an E6 protein of 137 amino acids that acts as a transcriptional activator, p53 and paxillin ligand, presenting also telomerase activity.
For the first time, BPV-2 E6 protein is expressed and purified in a bacterial system.
68 C [right arrow] T 134 G [right arrow] T 405 C [right arrow] A E6 BPV-2 Codon Ref.