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For the seven samples characterized this way, the fractions of IgL[kappa] expression calculated directly from the measured CT values, i.e., without correcting for sample-specific effects, were 52% (BR0), 59% (BR46), 60% (BR42), and 63% (BR37) in the negative samples and 24% (BR18), 43% (BR17), and 89% (BR52) in the positive samples (Table 1 in the Data Supplement).
Variations in CT values for the Ig[L.sub.k] and IgL[lambda]] reactions in eight repeated measurements of sample BR0 run either in parallel (intraassay) or separately (interassay).