Gene Primer seq 5'-3' gtfB F-AGCAATGCAGCCAATCTACAAAT R-ACGAACTTTGCCGTTATGTCA gtfC F-GGTTTAACGTCAAAATTAGCTGTATTAGC R-CTCAACCAACCGCCACTGTT ftf F-AAATATGAAGGCGGCTACAACG R-CTTCACCAGTCTTAGCATCCTGAA gbpB F-ATGGCGGTrATGGACACGTT R-TTTGGCCACCTTGAACACCT VicR F-TGACACCAUACAGCCITFGATG R-CGTCTAGTTCTCGTAACATTAACTCCAATA brpA F-GGAGGAGCTGCATCAGGATTC R-AACTCCAGCACATCCAGCAAG RelA F-ACAAAAAGGGTATCGTCCGTACAT R-AATCACGCTTGGTAUGCTAATTG spaP F-GAGGTAATGGTFATGCATCAA R-TITGTATCAGCCGGATCAAGTG 16S rRNA F-CCTACGGGAGGCAGCAGTAG R-CAACAGAGCTTTACGATCCGAAA Smu0630 F-GTTAGTTCTGGTTTTGACCGCAAT R-CCCTCAACAACAACATCAAAGGT F-forward; R-reverse.
Protein sequences for gtfC (BAA26102), gtfB (BAA26105), brpA (NP_720858) and spaP (NP_721042) were downloaded from NCBI database.
The binding pockets and active sites of selected structures (brpA, gtfB, gtfC and spaP) were identified by using POCKET finder programme (www.modelling.leeds.ac.uk/pocketfinder).
Sources said unfortunately the perception of intelligence agencies involvement in this tragic incident is being created by some members of few political parties and militant organizations like BLA, BRPA
, BLNF and BLF as part of their ongoing propaganda campaign.