BSTFAN,o-Bis (Trimethylsilyl) trifluoroacetamide (derivatization reagent)
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Abbreviations: BSTFA, N,O-bis(trimethylsily)trifluoroacetamide; El, electron ionization; FBS, foetal bovine serum; GC-MS, gas chromatography-mass spectrometry; NCI, National Cancer Institute; SRB, sulforhodamine B; TCA, trichloroacetic acid; TMCS, chlorotrimethylsilane; TMSi, trimethylsilyl.
About 5 mg of the extract were mixed with 50 pi of dry pyridine and 75 pi of BSTFA and heated at 80[degrees]C for 20 min.
Also, in order to protect the functional groups of the components, each sample was siliconized by using BSTFA (bis-[trimethylsilyl]-trifluoroacetamide).
To further improve resolution, we investigated the difference in chromatographic performance between BSTFA and propionic acid anhydride derivatized samples.
Extracts were reconstituted in acetonitrile and subjected to dual derivatization with MTBSTFA with 1% TBDMCS and BSTFA with 1% TMCS.
Summary: To analyze the fatty acid contents of Buddleja asiatica Lour,both the non-volatile oil and fat obtained from the n-hexane soluble sub- fraction were subjected to GC/MS using BSTFA (N,O- bis(trimethylsilyl) trifloroacetamide) derivatization.
After cooling, 20 [micro]L BSTFA + 1% TMCS was added, and the vials were crimp-capped and incubated 45 min at 80[degrees]C.
Derivatization prior to GC/MS analysis was performed with 250 [micro]l of BSTFA, vigorous vortexing and incubation at 70 [degrees]C for 20 min.
BSTFA (20 [micro]L containing 1% TMCS) was added, and the mixture heated for 15 min at 80[degrees]C.