All cultures were aerobically grown on trypticase soy agar slants (TSA) (BBL Microbiology systems, Cockeysville, MD) or BTJA at 44[degrees]C.
Cadaverine was added aseptically at levels of 0, 40, 80, and 120 mM to the sterilized BTJA medium.
The effect of cadaverine as a potential inhibitor was also observed on growth of isolates in BTJA. The effect of cadaverine was noted on the pH change of the BTJA.
With use of BTJA, this lack of pH elevation was always repeated.
Comparative Growth of Isolates in Acidified PBB and BTJA
These acidic products were added to bring PBB (Table 3) and BTJA to pH 4.3 before autoclaving.
Desired colonies were those that grew on BTJA and produced zones of purple in the yellow, acidified medium.
All cultures were grown aerobically on trypticase soy agar slants (TSA) (BBL Microbiology Systems, Cockeysville, MD) or BTJA at 44[degrees]C.
Moreover, the ability of isolates to proliferate in BTJA suggests that our isolates may be able to grow at the same pH as in ripe or overripe tomatoes that have relatively high digested carbohydrate content.
Therefore, the Rice and Pederson study supports the results of the present study in which tomato juice can be used as an acidulant in BTJA, with no inhibitors for aciduric spore-former Bacillus strains at pH 4.4 (Table 7).
The BTJA medium was challenged with the unidentified fungal isolate from soil samples.