Each plant sample was placed separately in a plastic bag and kept at 4[degrees]C until analyzed by Bean yellow mosaic virus (BYMV), Bean common mosaic virus (BCMV), Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Alfalfa mosaic virus (AMV) polyclonal antibodies, and DAS-ELISA was performed as described by Clark and Adams .
Single infection to BYMV, TSWV, BCMV and CMV was determined in 92, 81, 47 and 5 samples respectively.
BYMV isolates was efficiently transmitted to some plants by rub-inoculation.
In this study, faba bean samples are infected to BCMV, BYMV, TSWV and CMV.
BYMV isolate from Dezful, BYMV-D1 induced chlrotic and necrotic local lesion and then systemic symptoms on some species in chenopodiacea, leguminoseae and solanaceae families.
Because of difficulties in controlling TSWV vectors and the transmission of BYMV, BCMV and CMV by aphids in a non-persistent manner, these viruses can be responsible for the loss of up to 100% of faba bean crops.
Twenty-one plants were identified in Cycle 6 that survived field BYMV infection in combination with severe root rot disease.
The most striking response of arrowleaf clover to infection with BYMV is a rapid, systemic wilting beginning on the youngest growth 8 to 13 d postinoculation and resulting in plant death.
The development of BYMV tolerance was demonstrated with arrowleaf families from four cycles of selection where four BYMV disease symptoms (dwarfing, rugosity, chlorosis, and mosaic) and dry matter production were evaluated.