Suitable dilutions were spread plated at volumes of 0.1 mL on Baird-Parker agar
supplemented with egg yolk tellurite emulsion (Bioxon) and incubated under aerobic conditions at 37[degrees]C.
All swab samples were streaked on the selective Baird-Parker agar
and incubated at 37[degrees]C for 24 hr.
After incubation, samples were spread on Mannitol Salt Agar (Oxoid), Baird-Parker Agar
(Difco), Cetrimide Agar (Difco), MacConkey Agar (Difco), Eozine Methylene Blue Agar (Difco), Fluid Tetrathionate Medium (Difco), Xylose-Lysine Deoxycholate Agar (Difco), Bismuth Sulfite Agar (Difco) and Enterococcosel Agar (Difco) to determine the presence of specific microorganisms according to pharmacopeia (USP 2009).
After this procedure, 0.1 mL of the suspension was inoculated into Baird-Parker agar
(Himedia) for isolation of typical colonies of Staphylococcus.
0,1 ml of the dilution was thereafter spread-plated onto Baird-Parker Agar
with egg-yolk tellurite mulsion and incubated at 35[degrees]C for 24 hours according to the method by ISO .
The test utilizes the Baird-Parker Agar
(BPA) which is also commonly detailed in European and International Standards.
Microbiology of food and animal feeding stuffs-horizontal method for the enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus and other species): Part 1: technique using Baird-Parker Agar
One hundred [micro]L of the turbid TSB culture was also inoculated into TSB supplemented with oxacillin (2 mg [L.sup.-1]) and incubated overnight at 37[degrees]C with agitation (Jonas, Speck, Daschner, & Grundmann, 2002) prior to plating onto MSA and Baird-Parker agar
(Baird-Parker, 1962; Oxoid).
aureus ((Baird-Parker Agar
with EY Tellurite Enrichment), yeast and molds (Potato Dextrose Agar) and lactic acid bacteria (deMann Regosa Sharpe Agar) .
The Oxoid Brilliance Staph 24 Agar method has been validated and approved by MicroVal according to the ISO 16140 standard against the reference method ISO 6888:1999-Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)--Part 1: Technique using Baird-Parker Agar
medium for meat, dairy, seafood, bakery products and composite food.
aureus, we purified colonies that showed typical growth on MRSA Chrom-ID plates by transferring them to modified Baird-Parker agar
(7) and Columbia blood agar (Oxoid, Hampshire, UK) for DNAse and catalase testing.