The test utilizes the Baird-Parker Agar
(BPA) which is also commonly detailed in European and International Standards.
Microbiology of food and animal feeding stuffs-horizontal method for the enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus and other species): Part 1: technique using Baird-Parker Agar
1]) and incubated overnight at 37[degrees]C with agitation (Jonas, Speck, Daschner, & Grundmann, 2002) prior to plating onto MSA and Baird-Parker agar
(Baird-Parker, 1962; Oxoid).
Aliquots (1ml) were spread plated over the surface of Baird-Parker agar
(Oxoid, CM275), supplemented with egg yolk emulsion (Oxoid, SR047C).
The Oxoid Brilliance Staph 24 Agar method has been validated and approved by MicroVal according to the ISO 16140 standard against the reference method ISO 6888:1999-Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)--Part 1: Technique using Baird-Parker Agar
medium for meat, dairy, seafood, bakery products and composite food.
aureus, we purified colonies that showed typical growth on MRSA Chrom-ID plates by transferring them to modified Baird-Parker agar
(7) and Columbia blood agar (Oxoid, Hampshire, UK) for DNAse and catalase testing.
Oxoid Baird-Parker Agar
(ISO) is a selective diagnostic medium for the isolation and enumeration of coagulase-positive staphylococci in foods.
1 ml of each dilution of the sample was transferred to the surface of Baird-Parker agar
plates (g/L Distilled water; 63 g, Baird-Parker Medium (Oxoid CM275); 50 ml, SR54; pH 6.
Staphylococcus aureus counts were determined with Baird-Parker Agar
(Difco) with egg yolk--tellurite emulsion, and plates were incubated at 37[degrees]C for 24 hours to 48 hours.
Staphylococcus aureus was determined by spreading of 100 [micro]l from the appropriate dilutions on Baird-Parker agar
(Merck, 5406) and incubated at 37 [degrees]C for 48 h.
The Petriflim Staph Express Count plate method requires only one incubation temperature and is equivalent to the BAM three-plate Baird-Parker agar
and tube coagulase method.