A BeLPT drawn that day had a stimulation index of 2.0 (normal range, 1.2-2.0).
A BeLPT drawn 2 days prior was normal (stimulation index of 1.1).
BeLPT at that time was normal (stimulation index of 1.3).
We now know that cellular (rather than humoral) immunity is responsible for its manifestations, that increased susceptibility is conferred through major histocompatibility complex class II genetic variants, and that sensitization to beryllium can be detected via specific lymphocytic proliferative responses measured by the BeLPT (Amicosante and Fontenot 2006; Kreiss et al.
The abnormal BeLPT results at the time of acute illness indicate the presence of a cell-mediated immune response to beryllium concurrent with the acute reaction to beryllium.
However, only individuals who had bronchoscopy were used in the analysis describing the predictive power of radiographs or BeLPT.
Because the PPD reaction is similar to BeLPT, this suggests that a decreased immune response to beryllium may occur in individuals with a lower body burden of beryllium (i.e., antigen).
Only 56 of the 110 (51%) individuals who screened positive by radiograph or BeLPT elected to have a bronchoscopy.
Having two positive BeLPTs and scarring on the chest radiograph, involving either all zones or the lower zones only, had the highest predictive value for the development of CBD (100%).
A combination of two positive BeLPTs and an abnormal chest radiograph on the initial medical screening was the best predictor of the presence of CBD.
Cells were cultured at 1 x [10.sup.6] cells/mL in triplicate or quadruplicate according to the established BeLPT protocol (Rossman et al.
Using flow cytometry and the BeLPT, we demonstrated that the murine response to topical beryllium occurs in the LN draining the site of chemical application, the auricular LN in our study, and that T cells are activated and released into the peripheral blood.