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BMDMBone Marrow-Derived Macrophage
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BMDMBone Mineral Density Measurement
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Bone Marrow-Derived Macrophages. Macrophages are present in virtually all tissues.
Maze, "High-mobility group box 1 protein initiates postoperative cognitive decline by engaging bone marrow-derived macrophages," Anesthesiology, vol.
Primary Bone Marrow-Derived Macrophages (BMDMs) Differentiation and Polarization
Bone marrow-derived macrophages from [apoE.sup.-/-] or DKO background were cultured for 5 days with or without 0.1 or 0.6 [micro]M (10 or 50 ppb) NaAs[O.sub.2], after which cultures were challenged with 7-ketocholesterol for 24 h.
The question of how TRPM2 modulates LPS-ATPinduced pyroptosis of bone marrow-derived macrophage arises.
Therefore, we confirmed that K+ efflux acts on the upstream of caspase-1 and pyroptosis, and it is the requirement that TRPM2 regulates LPS-ATP-induced pyroptosis of bone marrow-derived macrophage.
(a) THP-1 macrophages and (b) bone marrow-derived macrophages were treated with 2 [micro]M R848 or CpG-2006 for 24 h.
Bone marrow-derived macrophages (BMDM) and aortic vascular smooth muscle cells (VSMCs) were isolated from [Casp3.sup.-/-][ApoE.sup.-/-] mice and treated with apoptosis inducers cycloheximide (CHX) (1 [micro]g/mL; 10 [micro]g/mL; 30 [micro]g/mL) and puromycin (PM) (1 [micro]g/mL; 10 [micro]g/mL; 30 [micro]g/mL), respectively, to validate their inability to undergo apoptosis.
Bone marrow-derived macrophages (BMDMs) were prepared by harvesting bone marrow from the femurs and tibias of 8-12-week-old C57BL/6 mice.
Therefore, we incubated murine bone marrow-derived macrophages (BMDM) alone, with untreated platelets, or with collagen-activated platelets and determined the effect of the platelets on the cytokine release.
Culture of Murine Bone Marrow-Derived Macrophages. Bone marrow cells were harvested from 8- to 10-week-old male C57/B6 mice (Charles River Laboratories Japan, Inc., Yokohama, Japan) by flushing the femur and tibia with RPMI 1640 medium.