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References in periodicals archive ?
Brain Heart Infusion (BHI) agar, Brain Heart Infusion (BHI) broth, Skirrow's supplement (SR69) Oxoid England, glycerol, cephalothin (30 [micro]g), and nalidixic acid (30 [micro]g) antibiotic disc were obtained from Mast Group Ltd., Merseyside, UK.
The sludge samples (1 mL) were inoculated in 2 mL of Brain Heart Infusion (BHI) broth which contained cefotaxime (2 mg/L) and then the sample was incubated at 37AdegC for two hours under aerobic conditions.
Subsequently, growth of this organism was seen on both a Sabouraud Dextrose, Brain Heart Infusion Agar (SABHI), and a Lowenstein-Jensen (LJ) slant.
The swabs were transferred to MRL in transport medium composed of brain heart infusion (BHI) broth supplemented with 1% glucose.
Key words: Fowl cholera, Layer farms, Polymerase chain reaction, Brain heart infusion blood agar, Pasteurella multocida.
Transconjugants were selected on brain heart infusion medium containing vancomycin (32 [micro]g/mL) and fusidic acid (25 [micro]g/mL).
Silver nitrate was purchased from Sigma-Aldrich and brain heart infusion media was purchased from Himedia laboratories, India.
The milk sample was subjected to cultural examination in BHI (Brain Heart Infusion) broth and Antibiotic Sensitivity Testing (ABST) in the laboratory.
2.5 ml of this liquid culture was added in two 5ml of Brain Heart Infusion Broths with sucrose, congo red and sterile human tooth one with 0.3% aspartame and other without aspartame and incubated at 37 [degrees]C with 10% C[O.sub.2] for 18 hrs.
A 20 ul of pre-culture suspension match with Mc-Farland 0.5 inoculated in glass tube containing 1 ml of Brain Heart Infusion broth plus 1 ml of tested compound of required concentration and then left this tube at an angle of 30 undisturbed for 18 hr at 37degC for culturing and adhesion.
Cultures grown overnight at 37[degrees]C in brain heart infusion (BHI) broth on a rotary shaker (150 rpm) and bacterial growth was checked by changes in turbidity after 24hrs.