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bDNABranched DNA (biochemistry)
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Significantly, the new probe design permits the detection of such targets without the disadvantages encompassed in competing high-sensitivity methods such as nucleic acid amplification-based detection and branched DNA (bDNA) probe technologies, which can involve high cost, high complexity, time consuming processes and disruptions of sample integrity.
DiaCarta offers research use and CE-IVD products and CLIA services for cancer gene mutation detection by leveraging different technology platforms, including single gene or small panel of genes using qPCR platform, multiple gene target detection using multiplex assays, and high-throughput next generation sequencing platforms powered by XNA technology or branched DNA technologies.
The most commonly used methods in clinical laboratories include PCR, nucleic acid sequence--based amplification (NASBA), (2) and branched DNA (bDNA) assays.
A SANAS-accredited private laboratory performed an HIV-1 RNA VL on plasma using the VERSANT HIV-1 RNA 3.0 assay (branched DNA) (Siemens Healthcare Diagnostics, USA).
This assay is based on branched DNA (bDNA) signal amplification technology and enables quantification of p53-like mRNA levels in as few as 2,500 hemocytes, with a coefficient of variation close to 6% (range, 2-12%).
Some of these include branched DNA, hybrid capture, transcription-mediated amplification, cleavase-based assays, home-brew fluorescence in situ hybridization assays (where the DNA of interest is ordered using public genomic databases and fluorescently labeled in house), Invader assay, strand displacement assays and pyrosequencing.
Hybrid capture and branched DNA assays are both types of signal amplification procedures.
They also showed that FAN1 cleaves branched DNA but leaves the normal, separate double-stranded DNA alone.
These tests, using polymerase chain reaction (PCR) or branched DNA (bDNA) technology, offered a more direct method of measuring the treatment effect on the virus in blood, as well as other tissue compartments, compared to measuring p24 antigen or CD4 cells.
Human immunodeficiency virus RNA was detected using a branched DNA amplification technique as described by Collins et al, (3) except detection was automated on the Ventana Gen II (Ventana Medical Systems).
These tests go by several different names, like PCR (polymerase chain reaction) or bDNA (branched DNA), but they all work roughly the same way.
The guidelines advise considering treatment in these patients if the plasma viral load of HIV RNA rises to 30,000 copies/mL, as measured by the branched DNA test.
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