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Abbreviations: [As.sub.2][O.sub.3], arsenic trioxide; AGT, average generation time; BrdU, bromodeoxyuridine; CCPI/RI, cell cycle proliferative index/replicative index; EMS, ethyl methanesulphonate; HD, high dose [As.sub.2][O.sub.3]; HPL, human peripheral lymphocytes; KCI, potassium chloride; LD, low dose [As.sub.2][O.sub.3]; MD, medium dose [As.sub.2][O.sub.3]; PDT, population doubling time; PHA, phytoheamagglutinin; SCE, sister chromatid exchanges
Patterns of bromodeoxyuridine incorporation and neuropeptide immunoreactivity during arm regeneration in the starfish Asterias rubens.
AHN = adult hippocampal neurogenesis; BrdU = bromodeoxyuridine; DCX = doublecortin; GFAP = glial fibrillary acidic protein; NeuN = neuronal nuclei; NPCs = neural progenitor cells; and PSA-NCAM = polysialylated neuronal cell adhesion molecule.
We quantified the IR-induced [G.sub.1] checkpoint by measuring the incorporation of bromodeoxyuridine 6-8 hr after 1.5-Gy or sham treatment (Figure 2A, left).
First, we examined proliferation rates in ECTs by quantifying the number of cardiomyocytes that had incorporated bromodeoxyuridine (BrdU) into their nuclei during DNA replication (S phase of the cell cycle; Figures 5(a) and 5(b)).
For determination of DNA synthesis (labeling indices), we implanted animals with osmotic minipumps (Rats: Altzet 2ML1; mice; Altzet 2001; both from Charles River UK Ltd, Margate, Kent, UK) containing bromodeoxyuridine (BRDU; 15 mg/mL in phosphate-buffered saline, pH 7.4) 7 days before termination.
Cells are treated with thymidine analogues such as bromodeoxyuridine (BrdU) to directly track transplanted stem cells.
After establishment of DES, eye drops containing either bromodeoxyuridine labeled mesenchymal stem cells (n = 9) or phosphate buffer solution (PBS) (n = 7) were topically applied once daily for one week.
We measured cell division rates using bromodeoxyuridine (BrdU; Sigma Chemical) incorporation with PI staining (Sigma Chemical), which allows calculation of potential doubling time ([T.sub.pot]), a measure of cell cycle time that takes growth fraction but not cell loss into account (10,11).
(16) treated CD-1 mice with a range of potent synthetic estrogens and compared the uterotrophic responses of the mice to the induced changes in the expression of lactoferrin and Per and in the incorporation of bromodeoxyuridine (BrdU) into the luminal epithelium of the uterus.
Recently, we corroborated increased uterine cell proliferation following dermal application of 4-MBC and OMC by demonstrating increased bromodeoxyuridine uptake (4,5).