CA IICarbonic Anhydrase Isozymes I and II
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The catalytic activities of the purified human CA I (hCAI), human CA II (hCAII), and bovine CA II (bCAII) isoforms were tested in the presence and absence of anti-CA antibodies by use of an electrochemical method based on measuring the rate of CO2 diffusion from a buffered NaHCO3 solution (22).
Pure CA I and CA II IgGs were prepared by an immunoabsorption method in which purified human CA I and CA II antigens (Sigma Aldrich) were attached to N-hydroxysuccinimide (NHS)-activated columns (HiTrap NHS-activated column; no.
The specificity of the antiserum was tested by dot blot and Western blot analyses, which showed no cross-reactivity with human CA I, CA II, [alpha]-amylase, or IgA.