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Quantification of pyramidal cells hippocampal CA3 region: Pyramidal cells were quantified by direct visual counting of viable neurons using a light microscope (Motic Image Plus 2.0, China) under the total magnification of 400X.
GY% of PANI onto Ch was found to be 110%, 200%, and 275% for CAI, CA2, and CA3, respectively.
Meanwhile, inactivating the CA3 increased approach behaviour to the conflict.
The hippocampus consists of three major subfields: the dentate gyrus (DG) and the Cornus Ammonis areas 1 and 3 (CA1 and CA3) subfields (Ammon's horns), forming a unidirectional network via a trisynaptic circuit.
We further compared the expression changes of PICK-1 and PSD-95 in the subregion of the hippocampus (DG, CA3, and CA1) with immunohistochemistry and semiquantitative measurement by optic density.
These delays were incorporated to observe the time course of any possible injuries to the CA3 subregion.
Electrophysiology recording in the mossy fiber-CA3 region suggested that there was a higher amount of neurotransmitter release from mossy fiber to CA3. This conclusion was supported by higher frequency and higher amplitude of spontaneous excitatory activities and lower number of stimulation failures and decreased pair-pulse ratio of evoked activities in Hr-fedmice.
250 [micro]m area was randomly selected in CA1, CA3, and CA4 subregions as well as upper blade of dentate gyrus from each of the selected 5 [micro] thick hippocampal section and unbiased quantification was done manually using ocular micrometer scale.
Additionally, patterns formed in the dorsal CA3 have been shown to be biased towards object representations, while ventral CA3 place cells encode contexts [43, 44].
In the hippocampus, BDNF-positive cells were mainly concentrated in the granular cell layer of the DG and in the pyramidal cell layer of the CA1, CA3 and CA4 regions (Figure 2).
The CA3 place cells the researchers recorded continued to form unique representations for each environment.
activity between males and the other experimental groups were found in dorsal hippocampal region: CA1 (p < .05), CA3 (p < .05) and dentate gyrus (p < .05).
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