MC was therefore studied in the ERa and anti-AR CALUX, in the presence or absence of different sources of S9.
CALUX cells were seeded into 96-well tissue culture treated polystyrene plates (VWR, Dietikon, Switzerland) at a density of 10,000 cells per well.
09]M) were used as positive controls for the AR and ER[alpha] CALUX, respectively.
CALUX is perfect for screening and monitoring for contamination and if it is found, the confirmation by GC/MS provides the identification of the chemicals that are present that is used for regulatory intervention.
This allows data to be reported more rapidly with the XDS CALUX bioassay.
Third-generation Ah receptor-responsive luciferase reporter plasmids: amplification of dioxin-responsive elements dramatically increases CALUX
bioassay sensitivity and responsiveness.
genotoxic or immunotoxic) estimated based on FFQs, CALUX assay-based evidence of exposure to estrogenic-, androgenic-, and dioxin-like compounds, Hb adduct levels, and MN frequencies.
No association was found between Hb adducts with MNMONO frequencies; however, fetal exposure to compounds detected by ER[alpha] CALUX and AR CALUX induced significant increases of MNMONO, possibly reflecting genetic damage accumulated during fetal development (Kirsch-Volders and Fenech 2001).
Dioxin-Like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX
Previous studies have demonstrated that, whereas the CALUX assay-determined and the GC/MS-determined TEQs are highly correlated when only the PCDD/PCDF fraction of serum is analyzed, the correlation dissipates when the PCB fraction is included (Van Wouwe et al.
When the GC/MS-determined TEQs do not correspond to CALUX-determined AhR activities, the question then becomes, what added information is the CALUX assay indicating about toxic potency?
The National Library of Medicine has developed the infrastructure to screen compounds on possible pharmacologic leads and to report the data in an easily accessible publically available format; this is part of the National Institutes of Health Molecular Libraries Roadmap initiative, The results for the screening of sEH in this system are available online (National Center for Biotechnology Information 2009); the AhR CALUX
bioassay is currently used in the same program.