2.5 Aryl hydrocarbon (AhR) CALUX
reporter gene assay
The correlation of AR CALUX
data with Hershberger assay resulted in a correlation coefficient of [r.sup.2] = 0.46 (P < 0.0001) indicating that correlation was not strong.
is perfect for screening and monitoring for contamination and if it is found, the confirmation by GC/MS provides the identification of the chemicals that are present that is used for regulatory intervention.
To address the potential role of metabolism in the endocrine activity of EAS, liver S9 and cofactors for the cytochrome P450 enzyme family were incorporated into the CALUX
bioassay as an exogenous activation system.
Third-generation Ah receptor-responsive luciferase reporter plasmids: amplification of dioxin-responsive elements dramatically increases CALUX
bioassay sensitivity and responsiveness.
Trigger values for investigation of hormonal activity in drinking water and its sources using CALUX
Dioxinlike activity, expressed as aryl hydrocarbon receptor (AhR)-mediated activation of the extractable lipid fraction from plasma, was determined through the DR CALUX
bioassay developed by BioDetection System (Murk et al.
(chemical-activated luciferase expression) assay adapted and validated for measuring TCDD equivalents in blood plasma.
For instance, in a study of reproductive-age women in Belgium (Pauwels et at 2000), serum concentrations of PCB congeners 118, 138, 153, and 180 were each positively correlated, with chemically activated luciferase expression (CALUX
) toxic equivalent (TEC)) values of 0.34 s r ?.
For example, 80 Belgian teenage boys living near an incinerator (a presumed dioxin exposure source) had later sexual maturity, including smaller TV, compared with boys in an unpolluted town; however, TV did not correlate with indirect [chemical-activated luciferase gene expression (CALUX
) assay] measures of dioxin exposure (Den Hond et al.
2008) Nuclear hormone AhR [EC.sub.50] receptors (24 hr) transfected in CALUX
cell lines (Hamers AhR [IC.sub.50] et al.
These investigators used a chemical-activated luciferase gene expression (CALUX
) assay to measure cumulative biological effects of agonistic or antagonistic AhR ligands in mixtures.