CALUXChemical-Activated Luciferase Expression
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George Clark] The XDS CALUX technology can be used to identify sources of dioxins.
Feedinfo News Service] XDS CALUX bioassay method 4435 not only measures DLCs in feed and food, but it is also validated by the US Environmental Protection Agency to be used for measuring emissions gas, dust, ashes and other cinders, soil sediment and other matrices.
This allows data to be reported more rapidly with the XDS CALUX bioassay.
Key words: AHR, air pollution, CALUX, complex mixtures, endocrine disruption, ER, PAH.
The distinctions in chemical composition between phase, site, and season were not reflected in the results of the CALUX assays, suggesting the aggregate biologic activity of the different congeners in each extract is similar.
However, the results for urban versus rural location were not discernible in the results of the highly sensitive CALUX bioassays.
These differences could reflect the biological interaction of multiple full AhR agonists, partial agonists, and antagonists quantified in the CALUX assay but overlooked in the GC/MS assay.
Previous studies have demonstrated that, whereas the CALUX assay-determined and the GC/MS-determined TEQs are highly correlated when only the PCDD/PCDF fraction of serum is analyzed, the correlation dissipates when the PCB fraction is included (Van Wouwe et al.
The National Library of Medicine has developed the infrastructure to screen compounds on possible pharmacologic leads and to report the data in an easily accessible publically available format; this is part of the National Institutes of Health Molecular Libraries Roadmap initiative, The results for the screening of sEH in this system are available online (National Center for Biotechnology Information 2009); the AhR CALUX bioassay is currently used in the same program.
Dioxin-Like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX.
genotoxic or immunotoxic) estimated based on FFQs, CALUX assay-based evidence of exposure to estrogenic-, androgenic-, and dioxin-like compounds, Hb adduct levels, and MN frequencies.
No association was found between Hb adducts with MNMONO frequencies; however, fetal exposure to compounds detected by ER[alpha] CALUX and AR CALUX induced significant increases of MNMONO, possibly reflecting genetic damage accumulated during fetal development (Kirsch-Volders and Fenech 2001).