The plasmid vector contained the soft rot resistant aiiA gene fused between the CaMV
35S promoter and the OCS terminator, the herbicide resistant Bar gene and other elements (Fig.
officinalis [[DELTA].sup.6]-desaturase gene down stream of the 35S CaMV
The vector contained the TuMV resistant TuMV-CP gene fused between the CaMV
35S promoter and the OCS terminator the herbicide resistant Bar gene and other elements (Fig.
1) by replacing the CaMV
35S promoter contained in pBI221 (Clonetech, Palo Alto, CA) with 2.0 kb of sequences upstream of the ATG start codon (pSEF/GUS and pSPRP/GUS) or 2.4 kb of sequences upstream of the ATG start codon (pSPRP2.4/GUS).
promoter is "naked" DNA, without the protein coat.
One cassette had the aroA:CP4 gene driven by the rice actin 1 promoter and the other by the CaMV
enhanced 35S promoter.
The 35S promoter isolated from cauliflower mosaic virus (CaMV
) is the most common promoter used in plant genetic engineering.
Two plasmids were used for cotransformation: pC822, containing the Xa21 coding sequence, and pROB5, containing the selectable marker gene hph driven by the CaMV
35S promoter (Tu et al., 1998).
The vector used was pB5/ 35Sbar (supplied by Aventis), which contains the bar gene, driven by the cauliflower mosaic virus (CaMV
) 35S promoter, and the bacterial kanamycin resistance gene (neo) (Fig.
In the recombinant plasmid pBI121-LIF, which containing the GUS gene and the selectable marker neomycin phosphotransferase II (nptII), LIF was placed under the control of the cauliflower mosaic virus (CaMV
) 35S promoter (Fig.
pMON25948 contains phbA, phbB, and phbC, each under the control of the CaMV
35S promoter and fused to the Arabidopsis ribulose biphosphate carboxylase (RuBisCo) small subunit la transit peptide, pMON25949 is identical except phbA was replaced with bktB.