Collagen I is the primary component of the bone extracellular matrix and its expression is regulated by the Cbfa1 transcriptional regulator [2, 5].
Bone regeneration using an acellular extracellular matrix and bone marrow mesenchymal stem cells expressing Cbfa1.
Although cell response to micrometer topography and in particular surface microgrooves has been reported, studies have often been limited to using stochastically patterned substrata and covering only a small range of microgroove geometrical values and the influence of this interesting microgeometry on the main osteoblast specific transcription factor Cbfa1 has been ignored.
To our knowledge there has not been a report in the literature on the fabrication of microgroove gradients and their application in the study of Cbfa1 expression, activation and osteoblast differentiation.
Antibodies to Cbfa1 (Runx2) were from Abcam, Cambridge, MA, USA, and anti-rabbit IgG conjugated to Alexa Fluor 594 from Invitrogen.
Cellular differentiation was illustrated by the visualization of Cbfa1 in osteoblasts attached on the microtextured surfaces.
It can be noted the presence of Cbfa1 mainly out of the nuclei at 72 h on all microfabricated topographies.
Four reports in the May 30 Cell and one scheduled for the July 1 Journal of Cellular Biochemistry describe the discovery that Cbfa1 is vital to bone-forming cells, or osteoblasts.
Olsen and his group came across Cbfa1 during a search for the genetic mutations that cause cleidocranial dysplasia syndrome (CCD), a rare human skeletal disorder.
As his team hunted for the CCD gene, Olsen heard that two other groups had created mice with a similar skeletal disorder by mutating the mouse Cbfa1 gene.
Owen of the Imperial Cancer Research Fund in London, describe mice that have mutations in one or both copies of Cbfa1.