CBMNCytokinesis-Block Micronucleus (biomonitoring)
CBMNCape Breton Museums Network (Canada)
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The present study therefore aimed to evaluate cigarette smoke induced genotoxicity using CBMN assay in rats thus to assess the genotoxic effect of passive smoking.
Three ml blood was poured into the lithium heparinized tubes and stored at 4AdegC for estimation of micronucleus frequency by CBMN assay.
The CBMN assay measures all cells including necrotic and apoptotic cells as well as the number of nuclei per cell to provide a measure of cytotoxicity and mitotic activity.
From these two assays analyzing chromosomal abnormalities, the CBMN assay clearly is easier than the SCE assay.
Limitations of the CBMN assay are (1) its dependency on the scoring person due to some subjectivity of counting despite strict evaluation criteria (within one data set the person should not change, ideally two persons score the same cells) and, due to the duration of microscopical evaluation, (2) a restriction of the study group size.
Nuestros resultados en este estudio muestran un efecto genotoxico dosis-dependiente del sevoflurano determinado mediante el ensayo CBMN tras haber corregido las dosis de sevofluorano que eran citotoxicas en las curvas de supervivencia (MTT) en las celulas PNT2.
Diferentes autores describen que los resultados obtenidos por el ensayo de MN (ensayo de CBMN y Cometa) se contradicen con los obtenidos mediante el ensayo de SCE (24).
In this study we applied the cytokinesis-block micronucleus (CBMN) cytome assay to investigate the antimutagenic properties of Cynara scolymus L.
The cytokinesis-block micronucleus (CBMn) assay is a biological dose estimates for unknown radiological exposures that evaluate the human population micronuclei.
The goal of the present study, which is being conducted as part of the NewGeneris project, is to assess early genetic effects in newborns, and hence their potential risk to develop childhood cancer, using a well-validated biomarker of cancer risk in adults, the cytokinesis block micronucleus (CBMN) assay.