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Pairwise alignment and comparison of a 1,185-nt coding region of the small (S) segment showed similar degrees of sequence identity between CBNV and rodentborne hantaviruses, ranging from 61.4% for HTNV 76-118 to 58.0% for TULV (Tula virus) M5302v.
Phylogenetic trees based on sequences of the full-length M segment and partial S and L segments, generated by the maximum likelihood and neighbor-joining methods using the GTR + I + G model of evolution, showed similar topologies supported by bootstrap analysis, in which CBNV was relatively distinct from rodentborne and other shrewborne hantaviruses (Figure).
Designing suitable primers for the amplification of CBNV presented unanticipated challenges.