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After 30 min, either vehicle (10% DMSO), 100 mg/kg carbenoxolone (CBNX) or 500mg/kg semipurified extract was orally administered.
Investigation on the role of NP-SH in the gastroprotective effect of EAMM was carried out by prechallenging the EAMM or CBNX (positive control) with NEM (NP-SH blocker).
Further, pretreatment with L-NAME significantly (p < 0.05) reversed the gastroprotective action of saline-pretreated EAMM and CBNX.