IgG was detected in 31 (38.2%) goats; no cattle had positive results.
Conventional IgM/IgG ELISAs were used for LASV (Corgenix, Broomfield, CO, USA) and CCHFV
(Vector-Best, Novosibirsk, Russia), and reagents for the EBOV IgM/IgG ELISA (infected/uninfected cell lysates) were prepared at the Rocky Mountain Laboratories (Hamilton, MT, USA) and validated with serum from experimentally infected monkeys.
strains currently constitute 7 evolutionary lineages, 1 of which (Europe 2) contains the prototype strain AP92, which was isolated in 1975 from Rhipicephalus bursa ticks collected from goats in Greece (2).
In the past, serologic evidence of CCHFV
infection was reported in India from animal serum samples collected in western border districts, southern regions, Maharashtra state, and Jammu and Kashmir state (12).
antigens: - cchfv
gn glycoprotein antigen - 100g - cchfv
nucleoprotein (np) antigen - 100g - denv envelope (e) protein antigen, serotype 1 specific, insect cell expressed - 100g - denv envelope (e) protein antigen, serotype 2 specific, insect cell expressed - 100g - denv envelope (e) protein antigen, serotype 3 specific, insect cell expressed - 100g - denv envelope (e) protein antigen, serotype 4 specific, insect cell expressed - 100g - denv envelope (e) protein antigen, serotype 2 specific, mammalian cell expressed - 100g - denv envelope (e) protein antigen, serotype 4 specific, mammalian cell expressed - 100g - denv virus-like particles (vlps), serotype 1 - 100g - denv virus-like particl
Antibodies against CCHFV
nucleoprotein were detected by using an in-house CCHF recombinant ELISA (14).
Virus de la fiebre hemorragica de Crimea-Congo (CCHFV
) Fue aislado por primera vez entre 1944 y 1945 en la Peninsula de Crimea y posteriormente en la Republica Democratica del Congo en 1956 (Figura 2), confirmandose posteriormente que los virus eran indistinguibles antigenicamente, pero fue solo hasta 1970 que se nombro como CCHFV
improved the survival rates in mice infected with CCHFV
, when the dosing commenced 2 days post-infection.
Our aim was to investigate the role of the migratory birds in spreading CCHFV
through attached ticks.
Although early serological studies revealed very few differences between strains of CCHFV
; however, nucleic acid sequence analysis has demonstrated extensive genetic diversity, particularly between viruses of different geographic regions.
LASVnegative samples were then tested for IgM to DENV, WNV, YFV, RVFV, and CHIKV before being transferred to the US Army Medical Research Institute of Infectious Diseases for additional testing for IgM to EBOV, MBGV, and CCHFV
. IgM-positive samples were tested for IgG by ELISA and/or confirmatory PRNT, depending on the volume of sample available.
The etiologic agent, CCHF virus (CCHFV
; family Bunyaviridae, genus Nairovirus), is maintained in enzootic cycles involving agricultural and wild animals and the vector, Hyalomma ticks.