CCTCCChina Center for Type Culture Collection
CCTCCConference on Current Trends in Computational Chemistry
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The 16S rRNA gene was amplified using the universal primers 27f and 1492r [21], and the PCR products were sequenced by Invitrogen Biotechnology in CCTCC, Wuhan, China.
isolated a fungal strain Pseudomonas aeruginosa CCTCC AB91095 [27].
EV71 strain CCTCC/GDV083 (ATCC VR-784) (China Center for Type Culture Collection, CCTCC) was propagated using RD cells.
Three cell lines, K562, A549, and S-180, were used in anti-tumor assay in vitro and obtained from China Center for Type Culture Collection (CCTCC).
Mouse GMCs (SV40 MES 13) and rat GMCs (HBZY-1) were purchased from the China Center for Type Culture Collection (CCTCC) and maintained in low glucose (5.6 mmol/L) Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (Hyclone) at 37[degrees]C and 5% CO2.
It showed that all these SNPs formed a single haplotype block with strong LD ([r.sup.2]>0.8), and two major haplotypes (AGCTG and CCTCC) were found in the experimental population with frequencies 50.26% and 46.34%, respectively.
Human fibrosarcoma HT1080 cells were obtained from China Center for Type Culture Collection (CCTCC), Wuhan, China.
Antimicrobial activity: The in vitro antimicrobial activities of the essential oil were evaluated against panel, which included laboratory control strains obtained from the China Center for Type Culture Collection (CCTCC): two Gram- negative bacteria (Pseudomonas aeruginosa CCTCC AB93066 and Escherichia coli CCTCC AB91112), two Gram-positive bacteria (Bacillus subtilis CCTCC AB93174 and Staphylococcus aureus CCTCC AB91093), two yeasts (Hansenula anomala CCTCC AY92046 and Saccharomy cescerevisiae CCTCC AY92042) and four moulds (Aspergillus niger CCTCC AF91004, Chaetomium globosum CCTCC AF 200039, Mucor racemosus CCTCC AF 93209 and Monascus anka CCTCC AF93208).
Acquisition of that structure was likely the result of a transposition process because it was bracketed by a 5-bp duplication of the target site (CCTCC) that might be likely considered as the signature of transposition.
The 3 strains (CCTCC M 2016589, BUM 63.4 and BUM 1700) of M.
aeruginosa CCTCC AB93066 is resistant to a wider range of heavy metals, the MICs of most heavy metals are much lower than QTF5, accounting for 0.97 mM (Pb), 1.57 mM (Cu), 0.83 mM (Co), and 1.70 mM (Ni).