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CD44Cluster of Differentiation 44
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CD44 is a multifunctional single-chain transmembrane glycoprotein encoded by single gene, which is highly heterogeneous.
CD44 regulates the apoptotic response and promotes disease development in chronic lymphocytic leukemia.
To identify the origin of these cells, we next detected the expression of BMSC markers CD34, CD44, and CD105 by fluorescence immunoassay.
Primers were designed according to the sequences of Oct-4, Rex-1, CD29, CD44, CD71, CD73, CD90 and GAPDH (internal control) from GeneBank.
The third passage of ADSCs was identified by flow cytometric analysis of CD29, CD44, CD90, and CD45 (Abcam, San Francisco, USA).[14] Suspensions of ADSCs were prepared at 1 x 106 cells/ml.
Posteriormente se diluyo el sedimento celular en 80 o 40 ml de tampon por 107 de celulas totales, se agrego 20 ml de MicroBeads CD44 (Miltenyi) o 10 ml de CD24-Biotina, se mezclo e incubo durante 15 minutos en la oscuridad a 4 [grados]C.
Structure of the regulatory hyaluronan binding domain in the inflammatory leukocyte homing receptor CD44. Mol Cell..
Discriminatory immunohistochemical staining of urothelial carcinoma in situ and non-neoplastic urothelium: an analysis of cytokeratin 20, p53, and CD44 antigens.
Flow cytometry was performed using markers of mouse MSCs (CD44 and CD90.2), hematopoietic stem cells (CD34), and leukocytes (CD45).
The expression of selected myeloid-specific markers (CD11c, CD13, CD14, and CD45) along with HSPC markers (CD38, CD133) or specific marker for cell adhesion/proliferation (CD44) was analyzed by flow cytometry.