We selected TP53, TOP2A,
CDK1, CCNB1, CDC20, CCNA2, NDC80, AURKA, BIRC5, CCNB2, KIF11, and MAD2L1, which with worse overall survival situation according to the Kaplan-Meier plotter.
Chen et al., "Selective small-molecule inhibitor reveals critical mitotic functions of human
CDK1," Proceedings of the National Acadamy of Sciences of the United States of America, vol.
Musgrove, "Targeting cyclin-dependent kinase 1 (
CDK1) but not CDK4/6 or CDK2 is selectively lethal to MYC-dependent human breast cancer cells," BMC Cancer, vol.
The following primary antibodies were used in this study: [alpha]-SMA, [alpha]1(I) procollagen, and fibronectin (Epitomics, San Francisco, CA, USA); TGF-[beta]RI, TGF-[beta]RII, PDGF-[beta]R, EGF-R, and Bcl-2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); cyclin A, cyclin B1,
CDK1, CDK2, Bax, pro-caspase-9, cleaved-caspase-9, pro-caspase-8, cleaved-caspase-8, pro-caspase-7, cleaved-caspase-7, pro-caspase-3, cleaved-caspase-3, full-length PARP-1, cleaved-PARP-1, and [beta]-Actin (Cell Signaling Technology, Danvers, MA, USA).
The following primer pairs for target genes and GAPDH were chosen from the Primer Bank website: Bcl-2: 5'-TAC CTG AAC CGG CAC CTG-3' and 5'- GCC GTA CAG TTC CAC AAA GG-3';
Cdk1: 5'- GGGTCAGCTCGCTACTCAAC-3' and 5'-AAGTTTTTGACGTGGGATGC-3'; p53: 5'-TGT GGA GTA TTT GGA TGA CA-3' and 5'-GAA CAT GAG TTT TTT ATG GC-3'; GAPDH: 5'-TCCTGCACCACCAACTGCTTAG-3' and 5'-GGCATGGACTGTGGTCATGAGT-3'.
Among the enriched genes,
CDK1 plays an important role in the cell cycle, while RB1 is a driver gene in several cancer types.
Bladder cancer gene panels used in the Cxbladder Detect urinary test were also examined (
CDK1, MDK, IGFBP5, HOXA13 and CXCR2).
They found that after its levels peaked during the day, two proteins,
CDK1 and FBXW7, interacted with REV-ERB?
miRNA miR function Regulation miR-15a Tumor suppressor Down miR-125b Targets p53, stress response miR-199b Oncogene activation miR-218 Tumor suppressor miR-31 Apoptosis, tumor suppressor Up miR-21 Fatty acid synthesis, apoptosis Up miR-452 Targets
CDK1 Down miRNA Tissue/cell type miR-15a Primary bronchial epithelial cells miR-125b miR-199b miR-218 miR-31 Normal and cancer lung cells miR-21 Human squamous carcinoma cells miR-452 Human alveolar macrophages miRNA Source miR-15a Schembri et al.
(87) In addition, interestingly, DNMT3A-R882H could form a complex with cyclin-dependent kinase 1 (
CDK1) with higher capacity than wild type DNMT3A, leading to increased
CDK1 protein and enhanced cell cycle progression, suggesting a gain-of-function as well.
With the treatment of ZER and ZER-NLC, MDA-MB-231 cell cycle arrest at G2/M phase is proposed to occur through increased expression of cyclin B1,
Cdk1, Cdc25C, and Cdc25B proteins [23].
The expression of miR-16 in human PCa is decreased compared with normal prostate tissues, and evaluation of cellular models has shown that miR-16 inhibits prostate tumor growth through expression regulation of such genes as cyclin-dependent kinase 1 (
CDK1) and CDK2, which are involved in controlling the cell cycle and proliferation [61].