Group I was injected with normal saline (10 ml/kg) as control, Group II received standard drug diclofenac sodium (10 mg/kg) while the remaining groups were injected with CFDL (50, 100 and 150 mg/kg i.p.), DS and HDS (5 and 10 mg/kg, i.p.) respectively.
Group 1 was treated with saline (10 ml/kg), group II was treated with [Tramadol.sup.R] (30 mg/kg i.p.) while the remaining groups were injected with CFDL (50, 100 and 150 mg/kg i.p.), DS and HDO (5 and 10 mg/kg, i.p.), respectively.
Group I was treated with normal saline (10ml/kg), group 11 with diclofenac sodium (10 mg/kg), rest of the groups were treated with CFDL, DS and HDS at the same dose as in case of analgesic activity.
The animals were treated with saline, diazepam (reference drug, 0.5 mg/kg i.p.), CFDL (50,100 and 150 mg/kg i.p.), DS and HDS (5 and 10 mg/kg, i.p.).
The acetic acid induced writing was markedly protected by CFDL as presented in Fig.
The CFDL significantly increased (p < 0.05) the latency time in tested mice showing the significant analgesic effect in all corresponding doses.
The anti-inflammatory effect of CFDL, DS and HDS were dose dependent and diclofenac was used as reference drug as demonstrated in Figs.
The CFDL was proved as mild sedative (p < 0.05) in treatment dose of 100 and 150 mg/kg compared to the isolated compounds (DS and HDS).
In our study, the CFDL fraction, DS and HDS exhibited a prominent inhibition of writhing reflux in acetic acid-induced abdominal constriction assay.
lotus (CFDL) and its isolated constituents Diospyrin (DS) (6) and 8-hydroxyisodiospyrin (HDS) (7) exhibited marked anti-nociceptive, anti-inflammatory and sedative effects.