CFLARCASP8 and FADD-Like Apoptosis Regulator (Caspase-8 gene, CASP-8; Fas-Associated Protein with Death Domain, FADD)
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The cervical cancer cell lines were transfected with a total of 100 pmol of CFLAR (c-FLIP) Silencer Select Pre-designed siRNA (siRNA ID: s229912, Ambion) or Silencer Select Negative Control #1 (Ambion) using Lipofectamine 2000 (Invitrogen).
Sequences for IMPDH1 and 2, 2 known pseudogenes, and CFLAR (CASP8 and FADD-like apoptosis regulator) were obtained from GenBank.
[4] Human genes: IMPDHI and IMPDH2, inosine monophosphate dehydrogenase 1 and 2; CFLAR, CASPS and FADD-like apoptosis regulator; ALASI, [delta]-aminolevulinate synthase 1; B2M, [beta]2-microglobulin; G6PD, glucose-6-phosphate dehydrogenase; HPRTI, hypoxanthine phosphoribosyltransferase 1; and HMBS, hydroxymethylbilane synthase (formerly PBGD, porphobllinogen deaminase).
Antiapoptosis genes such as CASP8 and FADD-like apoptosis regulator (CFLAR) are induced by PAXgene reagent (see Fig.