CFU-GEMMColony-Forming Unit-Granulocyte, Erythrocyte, Monocyte/macrophage, Megakaryocyte
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(a) CFU-GEMM, (b) BFU-E, (c) CFU-GM, and (d) total CFU counts after 14 days in culture, using cells cultured for 4 days under various conditions (n = 3, *p < 0.05).
As a result, addition of these cytokines significantly increased the numbers of total CFU-C and CFU-GEMM colonies (Figures 5(a) and 5(b)).
Interestingly, noncontact coculture of CMML-BMSCs and CB [CD34.sup.+] cells for only 3 days resulted in reduced CFU-GEMM numbers with a skewed differentiation toward the granulomonocytic lineage over the erythroid lineage, which is consistent with the granulomonocytic hyperplasia in patients with CMML.
After 14 days of culture in H4435 methylcellulose, colonies of CFU-GEMM, CFU-GM, and BFU-E derived from coculture with HD-BMSCs or CMML-BMSCs were enumerated.
(a), (b), and (c) BFU-E, CFU-GEMM, and CFU-GM colonies, respectively, analyzed in the colony assay (original magnification: x200).