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Of note, when colony composition was analyzed, no significant difference was observed in the CFU-GM growth (Figures 2(c) and 2(d)) between treated and untreated cells with the notable exception of IL-6 + TIMP-1 for mPB-derived [CD34.sup.+] cells (p < 0.01).
Regardless of HP application, the cocultured groups (H_M_NA, H_M_NA_HP, H_M_A, and H_M_A_HP) produced significantly more BFU-E and CFU-GM colonies compared with the HSPC single-culture groups (H and H_HP).
As shown in Figure 5, CFCs counts (CFU-E, CFU-GM, BFU-E, and CFU-mix) in the Fe group were significantly decreased compared to the control group (all P< 0.001), and this effect was significantly reversed by DFX and NAC compared to the Fe group (all P< 0.05).
Our earlier cryoinvestigations demonstrated that the recovery of pluripotent and committed hematopoietic progenitors (CFU-Sd12 and CFU-GM) in the presence of lower concentration of cryoprotective agent (5% vs.
We observed very similar patterns of BFU-E and CFU-GM and CFU-Meg in both EML/ProINS cells and EML/empty cells (Figure 3(c)).
4 Gy TBI significantly decreased CFU-gM number compared to the unirradiated mice: HW rescued such decline (Figure 4(a)).
The CFU-GM training system was made into 0.2 mi cell suspension (2 x [10.sup.5]), 0.6 ml horse serum, 0.2 ml GM-CSF and 0.7 ml methylcellulose (27 g/1) by well mixing, after that the mixed solution was adjusted to 2.0 ml by using RPMI 1640 nutrient solution.
BMMNCs were planted into 24-well plates (Costar, Corning, USA) containing the relevant CFU-GM semisolid culture medium.
 Nonstandard abbreviations: HSPC, hematopoietic stem and progenitor cell; MNC, mononuclear cell; rhP/Fc, recombinant human P-selectin/Fc chimera; HBSS, Hanks balanced salt solution; MRE, microrenathane tube; PE, phycoerythrin; sP, P-selectin; CFC, colony-forming cell; BFU-E, burst-forming unit, erythroid; CFU-GM, colony-forming unit, granulocyte-macrophage.
The response of CFU-GM to recombinant BMP-2 at concentrations of 0, 25, and 100 ng/mL from untreated cord blood is depicted in Figure 2.
After 14 days of culture in H4435 methylcellulose, colonies of CFU-GEMM, CFU-GM, and BFU-E derived from coculture with HD-BMSCs or CMML-BMSCs were enumerated.
In a comparison of samples from heart disease patients and healthy controls, lower numbers of CFU-GM were observed in the first group .
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- CFTR Associated Protein of 70 kDa