CGACTCenter for Genetics and Complex Traits (Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine; Philadelphia, PA)
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In detail, 5 [micro]g of total RNA was pooled from four individual animals within the same breed and then double-stranded cDNA containing the T7 RNA polymerase promoter sequence (5'-AAACG ACGGC CAGTG AATTG T AATA CGACT CACTA TAGGC GC-3') was synthesized using a TaKaRa cDNA Synthesis System Kit according to manufacturer's recommended protocol (TaKaRa, Dalian, China).
Different concentrations of hK2, autodigested hK2, phK2, hK2-ACT, hK2-PCI, hK2-PAI-1, hK2-protease inhibitor 6 (hK2-PI6), PSA, recombinant PSA (rPSA), PSA-ACT, CGACT, and ACT were measured in the assay to determine the relative reactivity ratios among the forms.