CHACCCentral Highlands Area Consultative Committee (Australia)
CHACCClassic and Historic Automobile Club of Caboolture (Australia)
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SEM images (Figure 3) showed over time the surface of the organoids to become smoother due increasing cell density and deposition of extracellular matrix, which bound the CHACC particles together forming a larger conglomerate.
Dead cells were barely observed on CHACC using UCM or BM MSCs, which demonstrated that the cell viability was not affected by CHACC microparticles.
CHACC has been shown to have excellent properties to function as a bone graft, but it lacks the key component required for autografts: living cells with osteogenic capacity.
In this study, UCM and BM MSCs were incorporated with CHACC microparticles to form organoids and their in vitro osteogenic potential was assessed and compared.
Although both BM and UCM MSCs were able to attach to CHACC microparticles, form organoids, proliferate, and differentiate down the osteogenic lineage, as expected, the BM MSCs showed higher levels of osteogenic differentiation than UCM MSCs.
Also, to utilise the angiogenic nature of UCM, MSCs with CHACC should be explored as well.
This study has shown that 200-300 fm CHACC granules can be a suitable carrier for human MSC proliferation and differentiation in vitro, for the purpose of injectable delivery opening up the use of CHACC for new applications within maxillofacial surgery and dentistry.
In their study, the researchers constructed CHACC and tested its physical and chemical properties using a number of microscopic and spectroscopic techniques.
The results showed that new bone formation was visible on the surface of the CHACC.