The samples were identified by MALDI-TOF/TOF MS (5800 MALDI-TOF/TOF/, AB SCIEX), Test method: laser source: 355 nm Nd:YAG, accelerating voltage: 2kV, positive ions; Automatic acquisition data, sample target: 384 opti-TOF123mm x 81 mmssabsciex, matrix: CHCA
, MS: 800-4000Da, selected parent ion(SNR>50) to MS/MS analysis.
For example, umbelliferone matrix obtained better results in the analysis of methamphetamine in hair than the common matrices CHCA
Cooperative Home Care Associates (CHCA
), a worker-owned cooperative in New York City, emphasizes both its provision of "quality home care to clients" and its "quality jobs for direct-care workers." (259) CHCA
is a certified B-Corps, meaning that it has made "a public commitment to considering the impact of their decisions on their employees, suppliers, community, consumers, and environment." (260) CHCA
has over 2,050 employees and brings in annual revenue of over $65 million.
Resulting peptides were then concentrated by using C18 Zip-Tips (Millipore) and eluted with 2 [micro]L of ChCA
matrix (66 [micro]L TFA, 0.1%; 33 [micro]L ACN) directly on an MTP AnchorChipTM 384 BC plate (Bruker Daltonics).
Supernatant thus obtained was smeared over thin film within different chambers present over a steel MALDI plate overlaid with CHCA
([alpha]-Cyano4-dihydroxybenzoic acid) in a ratio of 50:48:2 acetonitrile: water: trifluoroacetic acid solution (15-19-20).
A second application by the Cardiff Hackney Carriage Association (CHCA
) was refused.
GE Healthcare also secured its future growth through M&A, purchasing workforce management software provider API Healthcare, operating room management technology developer CHCA
Computer Systems, and certain Thermo Fisher Scientific Inc.
Each eluate (0.5 [mu]L) was immediately applied onto four wells of a 900 [mu]m [mu]Focus MALDI plate[TM] (Hudson Surface Technology, Inc., Fort Lee, NJ) with an equal volume of the o-cyano-4-hydroxycinnamic acid (CHCA
) solution containing methanediphosphonic acid (MDPNA).
The peptide samples were then mixed (1:1) with a matrix consisting of a saturated solution of CHCA
(a-cyano-4- hydroxycinnamic acid, Sigma) prepared in 50% ACN/0.1% TFA.
16 April 2014 - GE Healthcare, part of US technology and financial services conglomerate General Electric Company (NYSE:GE), said it had closed the acquisition of Canadian operating room (OR) management and analytics solutions provider CHCA
Computer Systems Inc for an undisclosed amount.
The extraction was vacuum-dried, redissolved in 0.1% trifluoroacetic acid, and mixed with [alpha]-cyano-4-hydroxycinnamic acid (CHCA
Both the standard peptide sample and the samples from H4-APPsw cell medium following immunoprecipitation (IP) were further processed by mixing 5 [micro]L sample with 5 [micro]L of 7 mg/mL MALDI matrix (a-cyano4-hydroxycinnamic acid, CHCA
, from Sigma-Aldrich, St.