Laboratories enrolled in CHPV performed well using all media and methods in the first 3 years of the CAP proficiency testing program.
Additionally, CHPV does not measure the clinical validity of the testing method, which is based upon sensitivity and specificity of the test in detecting clinically significant cancer precursors in a population of women.
Laboratories enrolled in the CHPV survey performed well, whether the challenge test result was positive or negative.
They were transported in plastic jars, identified, pooled, stored at -70[degrees]C until processed and tested for CHPV RNA by RT-PCR (13).
CHPV RNA was detected in both CSF and serum specimens of 2 cases.
CHPV RNA was detected in one of 2 pools of sandflies collected from affected locality in Khangaon village, Wardha district.
Sensitivity of different cell lines to CHPV: PP-9 cell line was observed as the most sensitive cell line to CHPV infection when a different study was carried out to determine the most sensitive cell line among PP-9 cells, Vero E6 and RD cell lines using IFA as the assay system.
However, a significant observation in the growth kinetics of CHPV in insect cell lines was that the eclipse period was not prominent (Fig.
In embryonated eggs also, CHPV could not be traced in the allantoic fluid.
Overall, different CHPV isolates were not very divergent from each other.
Importance of the amino acid substitutions in these proteins in the pathogenesis of CHPV infection remains to be determined.
Although several amino acid differences were recorded in G protein, the importance of these changes in the pathogenesis of CHPV infection needs to be determined.