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Responses of laboratories participating in the CHPV program from 2011 through 2014 were summarized and analyzed.
Overall participation in the CHPV program increased during the study period, with 502 laboratories participating in 2011, 571 laboratories participating in 2012, 630 laboratories participating in 2013, and 695 laboratories participating in 2014.
Nonidentity between the GPC amino acid sequences of AV B1030026 and TCRV was greater than the nonidentity between the GPC amino acid sequences of CHPV and SABV (Table), among the GPC amino acid sequences of the 5 viruses in group A (range 15.8%-23.7%), and between the GPC amino acid sequences of Latino virus (LATV) and Oliveros virus (20.6%).
Detection of CHPV RNA was done by RT PCR in acute serum samples and CSF according to the method described earlier (3).
They were transported in plastic jars, identified, pooled, stored at -70[degrees]C until processed and tested for CHPV RNA by RT-PCR (13).
The questionnaire requesting a summary of 2012 HPV testing data was distributed to all participating laboratories in the CAP PAP and CHPV programs in 2013.
CHPV (strain number 034627) used in the present study was isolated from Andhra Pradesh, India, from human serum in 2003 (1).
Growth kinetics in vertebrate cells: Cells were grown to approximately 90 per cent confluency in 24 well plates (Nunc, Denmark) and infected with CHPV at a multiplicity of infection (MOI) of 10.
During the outbreak investigations, 5 CHPV isolates were obtained in cell culture.
CHPV sequences representing 3 encephalitis cases, including 1 fatal case (patient 2, Table) and 2 febrile cases, were compared.
CHPV is designed specifically for cytology laboratories or other laboratories that perform HPV as their only viral testing method.
CHPV survey results from 2008 through 2010 were summarized and analyzed.
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