Methods: Distribution of allele and genotype frequencies of the muscle specific creatine kinase (CK-MM
) gene A/G polymorphism was assessed in a survey among 275 athletes residing in state of Isfahan (86 professional karatekas (43 male and 43 female) and 86 amateur karatekas (50 male and 36 female) and 103 non-athlete individuals (50 male and 53 female).
Although both the CK-MM
and CK-MB isoforms offer the potential to distinguish between DMD-affected and unaffected boys when used as biomarkers of DMD, the concentrations of CK-MM
in the serum of individuals with DMD are 10-fold higher than those of CK-MB (9, 10).
Figure 2 indicates that there was a marked decrease in all the variables (CK-MM
, CK-MB, LDH, and CK Total) with the HMB group demonstrating the greatest decrease across time.
pode ser mensurada na corrente sanguinea pelo fato de que com o estresse induzido pelo exercicio fisico ocasiona micro lesoes na estrutura das celulas, o CK-MM
acaba ultrapassando a membrana plasmatica, aparecendo assim no meio intramuscular.
values of the participants were found as 145.84[+ or -]74.29 U/L for PMAR, 199.68[+ or -]76.45 U/L for HT, 338.39[+ or -]177.40 U/L for AM, 742.54[+ or -]459.67 U/L for 24h, 402.66[+ or -]213.12 U/L for 48h and 254.23[+ or -]119.19 U/L for 72h.
The fact that electrophoresis shows 100% CK-MM
, however, suggests that skeletal muscle is the origin of increased CK activity.
The CK-BB isoenzyme migrates toward the anode at pH 8.6, in contrast to CK-MM
, which remains cathodic to the application point (Fig.
A CK e composta por um dimero composto de duas subunidades (B e M) que sao separadas em tres formas moleculares distintas: CK-BB ou CK-1 encontrada predominantemente no cerebro (Lang, Wurzburg, 1982); CK-MB ou CK-2, forma hibrida, predominante no miocardio e CK-MM
ou CK-3 predominante no musculo esqueletico (Apple, Hellsten, Clarkson, 1988).
There are three major CK isoenzymes; the two homodimers CK-MM
and CK-BB and the heterodimer CK-MB.
is found mostly in skeletal muscle, whereas CK-BB is found both in kidney and brain tissue.
Cytoplasmic CK is composed of M and/or B subunits that associate to form CK-MM
, CK-MB, and CK-BB isoenzymes.
Serum activities of CK-MM
may be increased in a number of conditions, including after strenuous exercise, inflammatory myopathies, infectious myopathies, dystrophinopathies, rhabdomyolysis, medications, metabolic myopathies, malignant hyperthermia, endocrine myopathies, and channelopathies.